Peer Review Record

Manuscript ID: JOSTA-202606-AC60

Article Title: Isolation and Identification of Blue Green Algae and Its Plant Growth Promoting Efficacy using Maize (Zea mays) Seed Germination Experiment

Article Type: Original Article

Double-anonymous review Accepted

Review Summary

ItemDetails
Submission Date15 June 2026
Editorial ScreeningCompleted
External Reviewers2
Review Rounds1
Revision TypeMajor Revision
Final DecisionAccepted
Acceptance Date12 July 2026

Editorial Assessment

The manuscript addresses a relevant area of applied microbiology by isolating cyanobacterial strains from sixteen maize-growing locations and evaluating their plant growth-promoting potential through a maize seed germination experiment. Reviewers recognised the effort involved in the isolation work and considered the topic pertinent to the understanding of cyanobacteria as plant growth-promoting microorganisms. The principal concern raised by both reviewers related to the reliability of taxonomic assignment, as identification to genus level based on morphological and biochemical characteristics alone was judged insufficient without molecular confirmation. Reviewers further noted that several characterisation tests applied are conventional bacteriological procedures of limited diagnostic value for cyanobacteria. Additional major revisions were requested concerning the description of the aqueous extract preparation, the adequacy of the seed germination experimental design and replication, quantitative assessment of phosphate solubilisation and siderophore production, stronger evidence for nitrogen-fixing capability, and the addition of further plant growth-promoting traits such as phytohormone production. Presentation required major revision, including compliance with the journal word limit, addition of keywords, consistent italicisation of scientific names, correction of citation formatting, complete figure and table legends, appropriate placement of all figures and tables, and replacement of low-resolution or incomplete images with high-quality figures covering all isolates and consortia evaluated. The authors satisfactorily addressed all comments, after which the manuscript was accepted for publication.

Review Timeline

Submission Received

15 June 2026

Manuscript submitted through the JOSTA submission portal.

Editorial Screening Completed

18 June 2026

Manuscript passed preliminary editorial checks.

Peer Review Initiated

28 June 2026

Two independent reviewers accepted the review assignment.

Reviewer Reports Received

01 July 2026

Reviewers recommended publication following major revision.

Revised Manuscript Received

10 July 2026

Authors submitted a revised version addressing reviewer concerns.

Final Decision

12 July 2026

Accepted for publication.

Published Online

19 July 2026

Article published in Volume 2, Issue 3.

Key Issues Addressed During Revision

Reviewer concerns are summarised by category. Specific comments and author responses are confidential.

CategoryNature of ConcernStatus
Taxonomic Identification Assignment of isolates to genus level considered insufficiently supported by morphological and biochemical data alone; established cyanobacterial identification criteria or molecular confirmation requested ✓ Addressed
Characterisation Methods Clarification requested on whether colony characters were described, and on the diagnostic value of conventional bacteriological tests (Gram staining, biochemical assays) for cyanobacterial identification ✓ Addressed
Microscopy & Image Quality Higher-resolution micrographs with scale bars and stated magnification required to resolve characteristic morphological features ✓ Addressed
Methodology – Extract Preparation Preparation of the cyanobacterial aqueous extract used for seed treatment to be described in full to ensure reproducibility ✓ Addressed
Experimental Design & Statistics Sample size of ten seeds per treatment queried; clarification of replication, experimental design and statistical analysis requested to support conclusions on germination and early seedling growth ✓ Addressed
Plant Growth-Promoting Traits Quantitative measurement of phosphate solubilisation and siderophore production requested in place of qualitative assessment; evaluation of additional traits such as phytohormone production suggested ✓ Addressed
Nitrogen Fixation Evidence Growth in nitrogen-free medium considered a screening step only; additional established evidence requested to substantiate the nitrogen-fixing claim ✓ Addressed
Citation Style In-text citation formatting to be corrected so that only the publication year appears in parentheses when author names form part of the sentence ✓ Addressed
Manuscript Length & Structure Manuscript exceeded the 4,000-word limit for research articles; keywords to be added following the abstract ✓ Addressed
Methods Clarity Isolation and screening procedures, and the morphological and biochemical characterisation subsection, to be described more clearly and in sufficient detail; an appropriate reference for the seed germination assay to be provided ✓ Addressed
Results Presentation Morphological characteristics in Table 1 to be described in the Results text; pH range for the Bromothymol Blue colour transition to be specified ✓ Addressed
Tables & Figures – Legends Legends or footnotes to be supplied for all tables and figures, with symbols (+, ++, +++, –) clearly defined and isolate names labelled in the relevant figures ✓ Addressed
Tables & Figures – Placement All figures and tables to be repositioned near their first citation within the appropriate section, including relocation of the figure discussed only in the Discussion ✓ Addressed
Completeness of Figures Results to be shown for all nine isolates and all three consortia; the missing siderophore production figure to be included; cropped Petri plate images to be replaced with complete, high-resolution images throughout ✓ Addressed
Language & Style Thorough revision for scientific clarity and language, including consistent italicisation of genus and species names, removal of unnecessary capitalisation, and consistent spacing throughout ✓ Addressed

Peer Review Statement

Journal of Sustainable Technology in Agriculture (JOSTA) employs a double-anonymous peer review process. The identities of authors and reviewers remain mutually confidential throughout the review and publication process. Reviewer reports and correspondence are not published.

Voluntary reviewer disclosure. JOSTA respects reviewers' autonomy regarding attribution. A reviewer who wishes to be identified in the published article may notify the editorial office at any point before final acceptance. Upon written consent, the reviewer's name and affiliation will be acknowledged in the Publication & Reviewer Details section of the article. Reviewers who do not provide consent remain anonymous and are listed as Anonymous. This policy is strictly voluntary; no reviewer is under any obligation to disclose their identity.

This page provides a transparent summary of the editorial and peer-review process in accordance with JOSTA's open-science commitments. It does not constitute a full disclosure of reviewer comments or author responses.