Peer Review Record

Manuscript ID: JOSTA-202608-C432

Article Title: Cold Plasma for Inducing Plant Defence Responses against Insect Herbivores

Double-anonymous review Accepted

Review Summary

ItemDetails
Submission Date09 August 2026
Editorial ScreeningCompleted
External Reviewers3
Review Rounds1
Revision TypeMinor Revision
Final DecisionAccepted
Acceptance Date29 September 2026

Editorial Assessment

The manuscript is a review synthesising cold-plasma biology, plant stress responses, plant defence pathways, and insect pest management. Reviewers found it fairly well written, with clean prose and few grammatical issues, but identified that the central focus was somewhat diffuse and recommended a clearer statement of scope and objective, along with a stated literature-search strategy appropriate to a narrative review. A key concern raised by multiple reviewers was the need to distinguish experimentally demonstrated mechanisms from proposed ones, and to avoid presenting increased antioxidant/defence-enzyme activity as direct evidence of insect resistance in the absence of insect-performance data. Reviewers also requested a more critical evaluation of the evidence linking each defence pathway to actual herbivore outcomes, incorporation of recent literature, a conceptual/graphical model linking plasma exposure to herbivore response, consistent terminology for the various cold-plasma and plasma-activated-water terms used, and a broader, more balanced concluding section that acknowledges variability, growth-defence trade-offs, and the largely observational nature of current evidence. The authors satisfactorily addressed all comments, after which the manuscript was accepted for publication.

Review Timeline

Submission Received

09 August 2026

Manuscript submitted through the JOSTA submission portal.

Editorial Screening Completed

14 August 2026

Manuscript passed preliminary editorial checks.

Peer Review Initiated

29 August 2026

Three independent reviewers accepted the review assignment.

Reviewer Reports Received

12 September 2026

Reviewers recommended publication following minor revision.

Revised Manuscript Received

21 September 2026

Authors submitted a revised version addressing reviewer concerns.

Final Decision

29 September 2026

Accepted for publication.

Published Online

30 September 2026

Article published in Volume 2, Issue 3.

Key Issues Addressed During Revision

Reviewer concerns are summarised by category. Specific comments and author responses are confidential.

CategoryNature of ConcernStatus
Scope & Focus Definition Scope and objective of the review clearly defined, sharpening the central focus, which had spanned cold-plasma biology, plant stress responses, plant defence, and insect pest management somewhat diffusely ✓ Addressed
Literature-Search Strategy Databases, keywords, period covered, and study-selection criteria stated for this narrative review ✓ Addressed
Introduction – Structure & Flow Paragraph structure and transitions revised, including reordering key sentences and providing a broader overview of alternative pest-management strategies before narrowing to cold plasma ✓ Addressed
Referencing Gaps Missing citations added in several sections lacking supporting references ✓ Addressed
Abbreviation Consistency Repeated re-abbreviation of ROS/RNS removed after their first definition in the Abstract ✓ Addressed
Mechanistic Evidence Distinction Direct insecticidal effects of cold plasma distinguished from indirect plant-mediated effects; experimentally demonstrated mechanisms distinguished from proposed ones; increased antioxidant/defence-enzyme activity no longer presented as direct evidence of insect resistance without corresponding insect-performance data ✓ Addressed
Critical Evaluation Strengthening Evidence linking each defence pathway to actual changes in herbivore behaviour, development, survival, or reproduction evaluated more critically, rather than describing pathways independently ✓ Addressed
Literature Currency Recent studies incorporated, including cold-plasma seed-treatment effects on herbivore resistance in soybean and sorghum-sudangrass, and plasma-activated water effects on spider mite populations ✓ Addressed
Field-Scale Evidence & Applied Considerations Discussion expanded to note that field-scale efficacy, economic feasibility, treatment standardisation, persistence, and compatibility with integrated pest management require substantially more evidence beyond current laboratory findings ✓ Addressed
Redundant/Generic Content Reduction Generic, textbook-like descriptions of conventional plant defence shortened to allow greater emphasis on the central research question ✓ Addressed
Conceptual Integration A conceptual/graphical model linking plasma parameters → ROS/RNS → plant signalling → defence traits → herbivore response added to strengthen conceptual integration ✓ Addressed
Content Expansion & Specificity Specific content added throughout, including named structural defences, defence-related genes, non-enzymatic metabolites, biological effects of cold plasma, clarified comparison basis for a terpenoid-related statement, and expanded discussion of the penultimate section ✓ Addressed
Conclusion Depth & Balance Conclusion substantially elaborated and rebalanced to acknowledge species- and pest-dependent variability, growth-defence trade-offs, and the largely observational (rather than gene-expression or molecular) nature of current evidence, closing with a call for more extensive studies to optimise cold plasma as a pest-management strategy ✓ Addressed
Terminology Consistency "Cold plasma," "cold atmospheric plasma (CAP)," "non-thermal plasma," "atmospheric cold plasma," and "plasma-activated water (PAW)" used consistently, with distinctions defined at first occurrence where the terms are not strictly interchangeable ✓ Addressed
Table Formatting Tables 1–3 checked for formatting, line wrapping, and spacing consistency, including missing spaces between words and citations ✓ Addressed
Language & Sentence Clarity Lengthy, multi-claim sentences broken into shorter statements; hedging language such as "suggests," "may contribute," and "is associated with" used where direct experimental evidence is not available, and minor word-choice corrections applied ✓ Addressed

Peer Review Statement

Journal of Sustainable Technology in Agriculture (JOSTA) employs a double-anonymous peer review process. The identities of authors and reviewers remain mutually confidential throughout the review and publication process. Reviewer reports and correspondence are not published.

Voluntary reviewer disclosure. JOSTA respects reviewers' autonomy regarding attribution. A reviewer who wishes to be identified in the published article may notify the editorial office at any point before final acceptance. Upon written consent, the reviewer's name and affiliation will be acknowledged in the Publication & Reviewer Details section of the article. Reviewers who do not provide consent remain anonymous and are listed as Anonymous. This policy is strictly voluntary; no reviewer is under any obligation to disclose their identity.

This page provides a transparent summary of the editorial and peer-review process in accordance with JOSTA's open-science commitments. It does not constitute a full disclosure of reviewer comments or author responses.